What is Magnet Assisted Transfection?
Magnet assisted transfection, or MagnetofectionTM, is a simple, highly effective method to transfect cells. It is an easy-to-use, fast and efficient technology that promotes the uptake of biomolecules, such as nucleic acids including plasmid DNA and siRNA, into cells using magnetic nanoparticles and magnetic fields.
An overview of the process:
- Biomolecules, such as nucleic acids including plasmid DNA and siRNA, are mixed with magnetic nanoparticles made of biodegradable iron oxides coated with cationic molecules in a simple one-step process to form a biomolecule / magnetic nanoparticle complex.
- The biomolecule / magnetic nanoparticle complex is added to the cells to be transfected in a standard multi-well plate.
- A magnetic force is applied beneath the cells to be transfected using a magnet array beneath the multi-well plate. This draws the biomolecule / magnetic nanoparticle complex onto cells on the bottom of the multi-well plate. The biomolecule is drawn towards, and delivered into, target cells, generally via endocytosis.
- This leads to rapid, efficient transfection without disturbing membrane architecture, causing chromosomal damage or leaving holes in cell membranes, therefore maintaining higher levels of cell viability. This is in stark contrast to physical transfection methods, including electroporation or biolistic methods, that can damage, create holes or electroshock the cell membranes causing cell death.
- The magnetic nanoparticles are biodegradable and non-toxic at the recommended doses.
- After delivery into the cells, the biomolecules are released into the cytoplasm by different mechanisms depending on the formulation used.
- nanoTherics magnefect technology is unique in that the magnetic field created is designed to oscillate, which promotes more efficient uptake of the biomolecule into the cell and results in transfection efficiencies higher than other transfection techniques.
Magnetofection is trademarked and the trademark is owned by Christian Bergemann