nanoTherics transfection technologies use magnetic nanoparticles and oscillating magnetic fields to accurately and rapidly transfect biomaterials into cells. Our proprietary magnet configurations and oscillating array systems providing a novel technique for improved transfection performance not used or available in traditional transfection devices.
How it works
- nTMag is an aqueous dispersion of magnetic nanoparticles coated with a positively charged polymer.
- DNA condenses onto surface of nTMag particle – many plasmids can condense onto a single nTMag particle.
- Magnefect-nano’s oscillating magnets pull nTMag-DNA particle complex onto cell surface. Mechanical stimulation of cells caused by oscillating motion of particles complex stimulates endocytosis, resulting in enhanced nTMag–DNA particle complex uptake.
- nTMag’s polymer coating facilitates particle complex escape from the endosome where nTMag particle and plasmid DNA are then released into the cytoplasm. Plasmid DNA can then diffuse into nucleus where it can be transcribed and translated into transgene product.
Right: Primary Cortical Neuron transfection on DIV 5
(Data Courtesy of Dr Elena Di Daniel, Principal Scientist, Takeda Pharmaceuticals, Cambridge, UK)