highly efficient transfection technology
The patented magnefect-nano™ system utilises a novel, oscillating magnet array that greatly improves the transfection speed and efficiency over commercial lipid-based and static magnetofection agents. Results so far indicate that our transfection devices are up to 100 times more efficient at short transfection times than the best lipid-based technologies on the market today. magnefect-nanoTM also delivers significantly higher cell viability compared to electroporation systems:-
Figure 1: Luciferase activity in NCI-H292 human lung epithelial cells transfected with pCIKLux luciferase reporter construct using OzBiosciences Polymag® particles (“No magnet”, “Static” and “Oscillating”), Lipofectamine2000 (“LF2000”) and GeneJuice (“GJ”). Transfections were performed in 96 well tissue culture plates using 0.1mg DNA/well with 2 hours transfection time. Data shown as mean ± SEM (n=12).
a technology for "difficult to transfect" cells
The high transfection efficacy of the magnefect nano™ system, affords the potential for its use in hard to transfect cells and tissues. The potential to penetrate physical barriers to transfection, such as mucous coated lung epithelial cells, make this technology a prime candidate for therapeutic applications in genetic disorders such as cystic fibrosis.
Transfection occurs very rapidly with the magnefect nano™ system, achieving higher levels of transfection in 20 minutes than the current Lipofectamine2000 transfection standard after 6hours
Importantly, the nanotherics magnefect nano™ system does not significantly affect cell viability, unlike other physical non-viral techniques such as electroporation, making it an ideal candidate for therapeutic applications and for high value, in vitro experimentation such as stem cell research, where cell viability is economically desirable.
Figure 2: GFP expression in HEK293T cells transfected with 150 nm Micromod Nanomagâ-D-SPIO PEI magnetic nanoparticles coated with pEGFPC1 DNA in response to (a) static magnetic fields, and (b) oscillating fields.